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The flow through was then measured via chromatographic analysis of chemically converted Dansyl-YVG into Dansyl-YV-NH2 or Dansyl-YVG(OH) YVG substrate into the Dansyl-YV- hydroxyglycine intermediate and the amidation product dansyl-YV-N2 by means of high-performance liquid chromatography (HPLC) as described by Ul-Hasan and colleagues [36]

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