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After the secondary antibody reaction, the bands were visualized with enhanced chemiluminescence, and the positive pixel area was detected by an image analysis system (Fusion, Germany)

Some people may experience minor digestive discomfort or allergies, particularly if they have underlying health issues

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A standard volume of master mix was added to each reaction tube, and Ub was added to initiate the ubiquitination reaction containing 100M Ub, 1M SKP1-FBXO22, 0.5M *NSD2 PWWP1, 0.5M compound, 0.1M UBA1, 1M UBE2R2, 1M UBE2D2 or 1M UBE2L3, and 1M ARIH1, 1M NEDD8~CUL1-RBX1, and 5mM MgATP in reaction buffer